§13 · feasibility map

The reachability map is the measured spinodal; the yes/no rides on a firewalled drive only (B) scores

A self-contained simulation maps only which switch a drive reaches: reachability is the measured spinodal, and the absolute flip-drive orders by measured promoter γ. It transfers to real autism-gene promoters — each is R19-bistable. Whether a given RNA dose flips a given neuron is a firewalled Δh; only (B), scored against held-out pre/post expression with no tuning, returns yes/no. [V]/[O].

FM1 maps 12 RNA-machinery switches from easiest (AGO2, spinodal 0.6223) to hardest (DGCR8, spinodal 0.7380); a drive at k = 1.05 flips just past the spinodal, k = 0.95 does not. FM2 extends the structure to 10 measured ASD promoters (SCN2A shallowest, PTEN deepest). FM4 shows the self-contained flip is a Δh-replay over all 22 switches, not evidence.

Reachability is the measured spinodal

A drive reaches a switch exactly when it crosses that switch's measured spinodal — there is nothing to assume. Across 12 RNA-machinery promoters the absolute flip-drive is monotone in γ, from AGO2 (easiest, spinodal 0.6223) to DGCR8 (hardest, spinodal 0.7380). A drive scaled to k = 1.05 of the spinodal flips it; the same drive at k = 0.95 does not. The map is geometry, not a prediction that any particular dose lands.

The structure transfers to real autism-gene promoters

The same read applies to 10 measured SFARI ASD-gene promoters: each is an R19 bistable switch, and the flip-drive orders by the measured promoter γ — from the shallowest (SCN2A, γ 1.1982) to the deepest (PTEN, γ 1.5694).

FM2 — measured ASD-gene promoters are R19 switches, flip-drive ordered by γ
geneγspinodalbarrierrole
SCN2A1.19820.50480.3589voltage-gated sodium channel Nav1.2 (ASD/epilepsy)
NRXN11.35050.60410.4560neurexin-1 presynaptic adhesion (ASD/schizophrenia)
CHD81.40800.64310.4956chromodomain helicase DNA-binding 8 (highest-confidence ASD risk)
NLGN31.43660.66280.5160neuroligin-3 postsynaptic adhesion (X-linked ASD)
SYNGAP11.44630.66950.5229synaptic Ras-GTPase-activating protein (ASD/ID)
FMR11.45630.67640.5302fragile-X mental retardation 1 (Fragile-X; translational brake)
MECP21.49510.70360.5588methyl-CpG-binding protein 2 (Rett; X-linked ASD, chromatin reader)
SHANK31.52070.72180.5781postsynaptic density scaffold (Phelan-McDermid ASD)
TSC21.54350.73810.5956tuberous sclerosis 2 / mTOR brake (ASD with TSC)
PTEN1.56940.75670.6158PI3K phosphatase (macrocephaly/ASD)

The honesty gate: a self-contained flip is replay, not evidence

By construction a flip exists at k ≥ 1 for every one of the 22 switches checked, and never at k < 1. That screen is a function of the assumed drive alone — it replays the firewalled Δh rather than testing it, so a self-contained simulation cannot return whether a real cell changed. The only honest crossing is (B): score the predicted flipped-switch set and ordering against held-out measured pre/post expression of real siRNA/saRNA-treated cells, with no tuning of Δh or parameters to the target. Until (B) is run, the per-neuron yes/no stays [O].

FIREWALL · R19-bistability and the γ-ordered flip-drive are MEASURED geometry; the absolute RNA Δh and the per-neuron yes/no are runtime [O], pending the (B) held-out crossing. Every clinical application — vaccination, gene therapy, fertility care — is handed to clinicians and regulators. see the [O] ledger →